FIGURE

Fig. 4

ID

ZDB-FIG-180912-76

Publication

Aman et al., 2018 - Wnt/β-catenin regulates an ancient signaling network during zebrafish scale development

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Fig. 4

An Fgf–Wnt/β-catenin signaling interaction is necessary for differentiation of scale forming cells.

(A,B) Imaging sp7:EGFP over a five day treatment with 1 µM BGJ398 to inhibit Fgf receptors revealed completely arrested squamation (n = 8), whereas DMSO-treated control fish developed normally (n = 8). (A’,B’) BGJ398 treatment also arrested growth of individual scales. (C) hag/+ mutants overexpress Fgf8a and had disorganized differentiation of sp7:EGFP+ dSFCs (n = 6). (D) dSFCs did not appear in hag/+ mutant skin with hs:dkk1 induction (n = 12). (E) Loss of Eda signaling in nkt/nkt mutants did not prevent differentiation of sp7:EGFP+ scale forming cells, likely owing to residual Wnt activity in the skin (n = 12). Scale bars, 1 mm (A–E); 100 µm (A’, B’).

Expression Data

Gene:	EGFP
Fish:	b1212Tg (AB) b1212Tg; w32Tg (AB) eda^{dt1261/dt1261}; b1212Tg (AB) fbxw4^dhi4000Tg/+; b1212Tg (AB) eda^{dt1261/dt1261}; fbxw4^dhi4000Tg/+; b1212Tg (AB) fbxw4^dhi4000Tg/+; b1212Tg; w32Tg (AB)
Conditions:	chemical treatment by environment: BGJ-398 heat shock
Anatomical Terms:	dermis scale primordium
Stage Range:	Days 7-13 to Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	b1212Tg (AB) b1212Tg; w32Tg (AB) eda^{dt1261/dt1261}; b1212Tg (AB) fbxw4^dhi4000Tg/+; b1212Tg (AB) eda^{dt1261/dt1261}; fbxw4^dhi4000Tg/+; b1212Tg (AB) fbxw4^dhi4000Tg/+; b1212Tg; w32Tg (AB)
Conditions:	chemical treatment by environment: BGJ-398 heat shock
Observed In:	scale anatomical structure development scale primordium scale primordium developmental growth scale primordium developmental process
Stage Range:	Days 7-13 to Days 30-44

Phenotype Detail

Acknowledgments

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