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ZIRC
ZFIN ID: ZDB-FIG-180911-18
Tanaka et al., 2018 - In vivo targeted single-nucleotide editing in zebrafish. Scientific Reports   8:11423 Full text @ Sci. Rep.
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to Prim-5

Fig. 5

The Target-AID system can generate nucleotide substitution mutants. Phenotypic representation of (A, lateral view) a wild-type embryo and (B, lateral view) a chdc.232C>T/c.232C>T F2 embryo at 24 hpf. The open arrow and open arrowhead indicate a smaller head and expanded blood islands, respectively. Phenotypic representation of (C, lateral view), (E, ventral view) a wild-type embryo and (D, lateral view), (F, ventral view) an oepc.178C>T/c.178C>T F2 embryo at 27 hpf. The arrowhead indicates a single eye in each respective panel. (G,H) Expression pattern of gsc in (G, lateral view) a wild-type embryo and (H, lateral view) an oepc.178C>T/c.178C>T F2 embryo at the shield stage. (I,J) Expression pattern of hgg1 in (I, dorsal view) a wild-type embryo and (J, dorsal view) an oepc.178C>T/c.178C>T F2 embryo at the tailbud stage. Scale bars represent 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ctslb AB standard conditions Bud prechordal plate ISH
tdgf1ko202/ko202(AB) standard conditions Bud Not Detected prechordal plate ISH
gsc AB standard conditions Shield shield ISH
tdgf1ko202/ko202(AB) standard conditions Shield shield ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
chrdko201/ko201(AB) standard conditions Prim-5 blood island increased size, abnormal
Prim-5 head decreased size, abnormal
tdgf1ko202/ko202(AB) standard conditions Shield shield gsc expression decreased amount, abnormal
Bud prechordal plate ctslb expression absent, abnormal
Prim-5 eye fused with eye, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.