FIGURE

Fig. 5

ID

ZDB-FIG-180911-18

Publication

Tanaka et al., 2018 - In vivo targeted single-nucleotide editing in zebrafish

Other Figures

Fig. 5

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Fig. 5

The Target-AID system can generate nucleotide substitution mutants. Phenotypic representation of (A, lateral view) a wild-type embryo and (B, lateral view) a chd^{c.232C>T/c.232C>T} F₂ embryo at 24 hpf. The open arrow and open arrowhead indicate a smaller head and expanded blood islands, respectively. Phenotypic representation of (C, lateral view), (E, ventral view) a wild-type embryo and (D, lateral view), (F, ventral view) an oep^{c.178C>T/c.178C>T} F₂ embryo at 27 hpf. The arrowhead indicates a single eye in each respective panel. (G,H) Expression pattern of gsc in (G, lateral view) a wild-type embryo and (H, lateral view) an oep^{c.178C>T/c.178C>T} F₂ embryo at the shield stage. (I,J) Expression pattern of hgg1 in (I, dorsal view) a wild-type embryo and (J, dorsal view) an oep^{c.178C>T/c.178C>T} F₂ embryo at the tailbud stage. Scale bars represent 100 μm.

Expression Data

Genes:	ctslb gsc
Fish:	AB tdgf1^ko202/ko202 (AB)
Anatomical Terms:	prechordal plate shield
Stage Range:	Shield to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	chrd^ko201/ko201 (AB) tdgf1^ko202/ko202 (AB)
Observed In:	blood island eye head prechordal plate shield
Stage Range:	Shield to Prim-5

Phenotype Detail

Acknowledgments

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