ZFIN ID: ZDB-FIG-180911-1
Messchaert et al., 2018 - Eyes shut homolog is important for the maintenance of photoreceptor morphology and visual function in zebrafish. PLoS One   13:e0200789 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 2

Immunohistochemistry on retinal sections of wild-type and eysrmc101/rmc101 zebrafish.

(A) Retinal sections of wild-type and eysrmc101/rmc101 zebrafish at 5 dpf and 5 mpf stained with antibodies against Eys (green) and centrin (red). (B) BODIPY (green) staining showing disorganization of photoreceptor outer segments in eysrmc101/rmc101 zebrafish (5 dpf and 5 mpf) compared to age- and strain-matched wild-type zebrafish (arrows). (C) Retinal sections of wild-type and eysrmc101/rmc101 zebrafish at 5 dpf (upper panel), 2 mpf (middle panel) and 5 mpf (lower panel) stained with antibodies against rhodopsin (green) and F-actin (red). Asterisks indicate mislocalization of rhodopsin to the inner segments and synapses of photoreceptor cells. (D) Retinal sections of wild-type and eysrmc101/rmc101 zebrafish at 5 dpf, 2 mpf and 5 mpf stained with antibodies against GNAT2 (green) and F-actin (red). Arrows indicate dysmorphic outer segments in mutant zebrafish. In all images, nuclei are counterstained with DAPI (blue). INL: inner nuclear layer; ONL: outer nuclear layer; OS: outer segments. Scale: 5 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
eys Ab1-eys TL standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-eys Adult eye photoreceptor cell photoreceptor connecting cilium IHC
Ab1-eys eysrmc101/rmc101(TL) standard conditions Day 5 Not Detected eye photoreceptor cell IHC
Ab1-eys Adult Not Detected eye photoreceptor cell IHC
rho Ab5-rho TL standard conditions Day 5 eye photoreceptor cell photoreceptor outer segment IHC
Ab5-rho Days 45-89 eye photoreceptor cell photoreceptor outer segment IHC
Ab5-rho Adult eye photoreceptor cell photoreceptor outer segment IHC
Ab5-rho eysrmc101/rmc101(TL) standard conditions Day 5 eye photoreceptor cell photoreceptor outer segment IHC
Ab5-rho Days 45-89 eye photoreceptor cell photoreceptor outer segment IHC
Ab5-rho Adult eye photoreceptor cell synapse IHC
Ab5-rho Adult photoreceptor inner segment layer IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-cetn IHC TL standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
IHC Adult eye photoreceptor cell photoreceptor connecting cilium
IHC eysrmc101/rmc101(TL) standard conditions Day 5 eye photoreceptor cell photoreceptor connecting cilium
IHC Adult eye photoreceptor cell photoreceptor connecting cilium
Ab2-gnat2 IHC TL standard conditions Day 5 eye photoreceptor cell photoreceptor outer segment
IHC Days 45-89 eye photoreceptor cell photoreceptor outer segment
IHC Adult eye photoreceptor cell photoreceptor outer segment
IHC eysrmc101/rmc101(TL) standard conditions Day 5 eye photoreceptor cell photoreceptor outer segment
IHC Days 45-89 eye photoreceptor cell photoreceptor outer segment
IHC Adult eye photoreceptor cell synapse
IHC Adult photoreceptor inner segment layer
Phenotype Details
Fish Conditions Stage Phenotype
eysrmc101/rmc101(TL) standard conditions Day 5 embryonic retina morphogenesis in camera-type eye decreased process quality, abnormal
Day 5 eye photoreceptor cell eys expression absent, abnormal
Day 5 eye photoreceptor cell photoreceptor outer segment malformed, abnormal
Day 5 photoreceptor cell outer segment organization decreased process quality, abnormal
Day 5 photoreceptor outer segment layer disorganized, abnormal
Days 45-89 embryonic retina morphogenesis in camera-type eye decreased process quality, abnormal
Days 45-89 eye photoreceptor cell photoreceptor outer segment malformed, abnormal
Days 45-89 photoreceptor cell outer segment organization decreased process quality, abnormal
Days 45-89 photoreceptor outer segment layer disorganized, abnormal
Adult embryonic retina morphogenesis in camera-type eye decreased process quality, abnormal
Adult eye photoreceptor cell eys expression absent, abnormal
Adult eye photoreceptor cell photoreceptor outer segment malformed, abnormal
Adult eye photoreceptor cell synapse ab2-gnat2 labeling mislocalised, abnormal
Adult eye photoreceptor cell synapse rho expression mislocalised, abnormal
Adult photoreceptor cell outer segment organization decreased process quality, abnormal
Adult photoreceptor inner segment layer ab2-gnat2 labeling mislocalised, abnormal
Adult photoreceptor inner segment layer rho expression mislocalised, abnormal
Adult photoreceptor outer segment layer disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One