FIGURE

Fig. 3

ID

ZDB-FIG-180827-15

Publication

Ferrero et al., 2018 - Embryonic Microglia Derive from Primitive Macrophages and Are Replaced by cmyb-Dependent Definitive Microglia in Zebrafish

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Fig. 3

Embryonic and Adult MG Have Distinct Origins

(A) Scheme of the transgenic lines used to address the MG potential of primitive versus definitive hematopoiesis.

(B and C) Whole-mount fluorescence microscopy images of Tg(kdrl:Cre; ßactin:Switch-DsRed; mpeg1:GFP) triple-transgenic embryos show absence of DsRed expression (red) in GFP⁺ pMFs (green) on the yolk ball at 30 hpf (B), and in GFP⁺ embryonic MG (green) in the brain parenchyma at 3 dpf (C) (n = 14).

(D) In contrast, in sections of adult triple-transgenic animals, DsRed labeling is observed in all GFP⁺ MG cells.

(E) Flow cytometry analysis on a brain dissected from a 12-week-old triple-transgenic animal identifies blood-derived mpeg1:GFP^high CNS-associated MFs (blue circle, MFs) and mpeg1:GFP^low MG (red circle, MG). Both populations express the DsRed reporter at high level (n = 5). Scale bar: 50 μm. MFs, macrophages; MG, microglia.

See also Figures S4 and S5.

Expression Data

Genes:	DsRedx EGFP
Fish:	gl22Tg; s898Tg; sd5Tg
Anatomical Terms:	ball macrophage brain macrophage
Stage Range:	Prim-15 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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