FIGURE

Fig. S3

ID

ZDB-FIG-180628-7

Publication

Kumar et al., 2018 - Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme

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Fig. S3

Cilia are present in the olfactory bulbs, neuromasts and otic vesicles of pam^-/- embryos

Confocal immunofluorescence images of the olfactory bulb (A, B), neuromast mechano-sensory cells (C, D), and otic vesicles of control and pam^-/- embryos probed with antibody against acetylated tubulin. Electron micrographs (G, H) revealed that at 6 dpf both kinocilia (KC) and actin-based stereocilia (SC) were evident emanating from the sensory hair cells in the otic vesicles of pam^-/- embryos; these structures assemble early in development at ~16 hpf. The insets in (A, B) show that the olfactory motile cilia have normal 9+2 ultrastructure. Bars = 5 μm (A-F), 1 μm (G, H) and 100 nm (insets in A and B).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pam^mbg5/mbg5
Observed In:	olfactory bulb motile cilium otic vesicle kinocilium otic vesicle stereocilium
Stage:	Day 6

Phenotype Detail

Acknowledgments

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