ZFIN ID: ZDB-FIG-180501-17
van der Vaart et al., 2017 - Mecp2 regulates tnfa during zebrafish embryonic development and acute inflammation.. Disease models & mechanisms   10(12):1439-1451 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Days 7-13

Fig. 3

Expression of central inflammatory cytokines is dysregulated in mecp2-null larvae. (A,B,C) qPCR was performed to determine the whole-organism expression of tnfa from 4 hpf to 7 dpf (A), and il1b and il10 from 1 dpf to 7 dpf (B,C), in wild-type or mecp2-null zebrafish. Gene expression is related to the expression of the housekeeping gene tbp, where the fold change relative to gene expression in 1 dpf wild-type embryos is shown (n=3 with 20 embryos or larvae pooled per sample for 1-7 dpf; 30 embryos were pooled per sample for the 4-12 hpf time points; data are representative of two individual experiments). One-way ANOVA with Tukey's post hoc test was used for all statistical analyses (***P<0.001; **P<0.01; *P<0.05; ns, not significant). (D-K) Representative confocal micrographs of 3 dpf Tg(tnfa:eGFP) wild-type and mecp2-null larvae showing the eGFP expression pattern in brain regions in a lateral view (D,H), brain regions in a dorsal view (E,I), posterior gut epithelium in a lateral view (F,J) and dorsal root ganglion neurons in a lateral view (G,K).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP mecp2fh232/fh232 ; pd1028Tg standard conditions Protruding-mouth Not Detected brain IFL
Protruding-mouth Not Detected dorsal root ganglion IFL
Protruding-mouth eye IFL
Protruding-mouth Not Detected gut epithelium posterior region IFL
pd1028Tg standard conditions Protruding-mouth brain IFL
Protruding-mouth dorsal root ganglion IFL
Protruding-mouth eye IFL
Protruding-mouth gut epithelium posterior region IFL
il1b WT standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
mecp2fh232/fh232 standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
il10 WT standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
mecp2fh232/fh232 standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
tnfa WT standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
mecp2fh232/fh232 standard conditions Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Day 5 whole organism RTPCR
Day 6 whole organism RTPCR
Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mecp2fh232/fh232 standard conditions Prim-5 whole organism tnfa expression decreased amount, abnormal
Long-pec whole organism tnfa expression decreased amount, abnormal
Protruding-mouth whole organism tnfa expression decreased amount, abnormal
Day 4 whole organism tnfa expression decreased amount, abnormal
Day 5 whole organism il10 expression increased amount, abnormal
Day 5 whole organism il1b expression increased amount, abnormal
Day 5 whole organism tnfa expression decreased amount, abnormal
Day 6 whole organism il10 expression increased amount, abnormal
Day 6 whole organism il1b expression increased amount, abnormal
Day 6 whole organism tnfa expression decreased amount, abnormal
Days 7-13 whole organism tnfa expression decreased amount, abnormal
mecp2fh232/fh232 ; pd1028Tg standard conditions Protruding-mouth brain GFP expression absent, abnormal
Protruding-mouth dorsal root ganglion GFP expression absent, abnormal
Protruding-mouth gut epithelium posterior region GFP expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.