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ZIRC
ZFIN ID: ZDB-FIG-180425-26
Wang et al., 2017 - Tet1 facilitates hypoxia tolerance by stabilizing the HIF-α proteins independent of its methylcytosine dioxygenase activity. Nucleic acids research   45(22):12700-12714 Full text @ Nucleic Acids Res.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage Range: Prim-5 to Days 45-89
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Prim-5 to Adult

Fig. 2

Knockout of tet1 in zebrafish causes more apoptotic cells in the brain and reduction of hypoxia-inducible gene expression under hypoxia. (A) Compared with those in the brain of wild-type siblings, more apoptotic cells were detected in the brain of tet1-null zebrafish by TUNEL assays under hypoxia (5% O2, 5 h). (B) Expressions of hypoxia-inducible genes including phd3, glut1, vegfa were reduced significantly in the brain of tet1-null zebrafish embryos and adults compared with those of their wild-type siblings under hypoxia. (C) Overexpression of both zebrafish tet1 and its enzymatic inactive form (zf-tet1-mut) could activate the hypoxia response element luciferase (HRE-luc.) reporter in the N2a cell line under hypoxia. N2a cells were transfected with the indicated plasmids and luciferase activity was measured 20–24 h after transfection. The left red box indicates the alignment of partial Tet1 sequences surrounding Tet1 enzymatic activity region and the enzymatic inactive mutant (mut) with histidine mutated to tyrosine and aspartic acid mutated to alanine. zf-tet1-mut, zebrafish tet1 mutant; mu-Tet1-mut, mouse Tet1 mutant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egln3 AB hypoxia Prim-5 brain RTPCR
Long-pec brain RTPCR
Days 45-89 brain RTPCR
tet1ihb2017/ihb2017(AB) hypoxia Prim-5 brain RTPCR
Long-pec brain RTPCR
Days 45-89 brain RTPCR
slc2a1a AB hypoxia Prim-5 brain RTPCR
tet1ihb2017/ihb2017(AB) hypoxia Prim-5 brain RTPCR
vegfab AB hypoxia Long-pec brain RTPCR
Days 45-89 brain RTPCR
tet1ihb2017/ihb2017(AB) hypoxia Long-pec brain RTPCR
Days 45-89 brain RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB hypoxia Prim-5 brain egln3 expression increased amount, abnormal
Prim-5 brain slc2a1a expression increased amount, abnormal
Long-pec brain egln3 expression increased amount, abnormal
Long-pec brain vegfab expression increased amount, abnormal
Days 45-89 brain vegfab expression increased amount, abnormal
Days 45-89 brain egln3 expression increased amount, abnormal
tet1ihb2017/ihb2017(AB) hypoxia Prim-5 brain egln3 expression amount, ameliorated
Prim-5 brain slc2a1a expression amount, ameliorated
Long-pec brain egln3 expression amount, ameliorated
Long-pec brain vegfab expression amount, ameliorated
Days 45-89 brain vegfab expression amount, ameliorated
Days 45-89 brain egln3 expression amount, ameliorated
Adult brain apoptotic process increased occurrence, abnormal
Adult response to hypoxia physical quality of a process, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nucleic acids research for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nucleic Acids Res.