FIGURE

Fig. 3

ID

ZDB-FIG-180425-14

Publication

Athanasiadis et al., 2017 - Single-cell RNA-sequencing uncovers transcriptional states and fate decisions in haematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Cells within distinct states have different repopulation potentials. a Experimental strategy for the adult transplantation experiment. Kidneys were dissected from transgenic donor fish and sorted for cells expressing the fluorescent transgene. Positive cells were collected and injected into sublethally irradiated rag2 ^{E450fs−/−} fish. b Assessment for engraftment was made 1 day, 4- and 14 weeks post transplantation using flow cytometry. Successfully engrafted fluorescent donor cells were isolated at 4 weeks PI by index sorting single cells into a microtitre plate for subsequent RNA-seq analyses. c Distribution of runx1+ cells, from non-transplanted (left) and transplanted fish at 4 (middle) and 14 wpt (right), modelled by Monocle

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.