PUBLICATION
Single-cell RNA-sequencing uncovers transcriptional states and fate decisions in haematopoiesis
- Authors
- Athanasiadis, E.I., Botthof, J.G., Andres, H., Ferreira, L., Lio, P., Cvejic, A.
- ID
- ZDB-PUB-171213-7
- Date
- 2017
- Source
- Nature communications 8: 2045 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Lineage/genetics
- Erythroid Cells/cytology
- Erythroid Cells/metabolism
- Gene Expression Profiling/methods*
- Gene Ontology
- Hematopoiesis/genetics*
- Humans
- Sequence Analysis, RNA/methods*
- Single-Cell Analysis/methods*
- Zebrafish/blood
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- PubMed
- 29229905 Full text @ Nat. Commun.
Citation
Athanasiadis, E.I., Botthof, J.G., Andres, H., Ferreira, L., Lio, P., Cvejic, A. (2017) Single-cell RNA-sequencing uncovers transcriptional states and fate decisions in haematopoiesis. Nature communications. 8:2045.
Abstract
The success of marker-based approaches for dissecting haematopoiesis in mouse and human is reliant on the presence of well-defined cell surface markers specific for diverse progenitor populations. An inherent problem with this approach is that the presence of specific cell surface markers does not directly reflect the transcriptional state of a cell. Here, we used a marker-free approach to computationally reconstruct the blood lineage tree in zebrafish and order cells along their differentiation trajectory, based on their global transcriptional differences. Within the population of transcriptionally similar stem and progenitor cells, our analysis reveals considerable cell-to-cell differences in their probability to transition to another committed state. Once fate decision is executed, the suppression of transcription of ribosomal genes and upregulation of lineage-specific factors coordinately controls lineage differentiation. Evolutionary analysis further demonstrates that this haematopoietic programme is highly conserved between zebrafish and higher vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping