FIGURE

Fig. S6

ID

ZDB-FIG-180420-62

Publication

Raghupathy et al., 2017 - Rpgrip1 is required for rod outer segment development and ciliary protein trafficking in zebrafish

Other Figures

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Fig. S6

Immunostaining of retinal sections of wildtype and rpgrip1^-/- zebrafish using anti-RPGR antibody showed abnormal localization of Rpgr. Rpgr was localized to the connecting cilia and outer segments of photoreceptors in 14dpf wildtype zebrafish retina, while Rpgr was mislocalized in inner segments of photoreceptor cells of rpgrip1^-/- mutant retinas and the fluorescence signals were significantly decreased. Arrow shows rod outer segment. (B) There is no difference in GRK7a localization to the cone outer segments in both wildtype and rpgrip1 mutant zebrafish at 5dpf. (C)The fluorescence signals of the boxed areas of wildtype and mutant retinal sections were measured using Image J software. There is no significant difference between wildtyep and mutants with p=0.6989. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium. NS, no significance.

Expression Data

Gene:	grk7a
Antibodies:	Ab1-grk7a Ab1-rpgr
Fish:	WT rpgrip1^fh314/fh314
Anatomical Terms:	eye photoreceptor cell ciliary transition zone eye photoreceptor cell photoreceptor outer segment retinal cone cell photoreceptor inner segment retinal cone cell photoreceptor outer segment
Stage Range:	Day 5 to Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rpgrip1^fh314/fh314
Observed In:	retinal cone cell photoreceptor inner segment
Stage:	Days 14-20

Phenotype Detail

Acknowledgments

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