Mislocalization of ciliary proteins in rpgrip1 mutant fish. (A) Rab8 was mainly localized to the connecting cilium of rod cells and some weak fluorescence signals were present in the outer segments of photoreceptor cells in wildtype (WT) zebrafish at 2 wpf; it was mislocalized in inner segments of photoreceptor cells and the localization to the outer segments of photoreceptor cells was lost in rpgrip1 mutant zebrafish at 2 wpf. (B,C) Transducin and GRK1 exhibited abnormal distribution in cone outer segments and loss of localization to rod outer segment, because the rod outer segments were absent in rpgrip1 mutant zebrafish. INL, inner nuclear layer; ONL, outer nuclear layer. (D) Quantification of fluorescence intensity of Rab8, GNAT1 and GRK1 signals. The fluorescence intensity was measured within three outer segment areas (25 × 25 µm²/area) in each section (four sections from four individual retinas were used) by Zen (Zeiss) at the condition of same laser intensity and master gain, and the data was analysed by t test with Graph Prism. The result is shown as mean ± SD. SD: standard deviation. p = 0.0027 (Rab8); p = 0.0084 (GNAT1); p = 0.0164 (GRK1).
