FIGURE

Fig. 2

ID

ZDB-FIG-180413-23

Publication

Dunn et al., 2017 - Dual Roles of Fer Kinase Are Required for Proper Hematopoiesis and Vascular Endothelium Organization during Zebrafish Development

Other Figures

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Fig. 2

Fer knockdown results in apparent vasculature blockage and accumulation of developing hematopoietic cells. At 30 somites, fer-MO1 embryos exhibit convergence extension defects along the anterior–posterior axis with red blood cells that are pooled in the posterior ICM region (A) with none present in the pumping heart (B), when compared to wildtypes ((C,D) respectively). By 48 hpf, the lack of circulating red blood cells and empty heart chambers is emphasized in the Fer knockdown embryos (E,F) compared to wildtype embryos (G,H). Agarose gel electrophoresis of fer-reverse transcription PCR from fer-MO1 injected embryos to demonstrate the blockage of Fer splicing at exon 9 (I).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-fer
Knockdown Reagent:	MO3-fer
Observed In:	circulating cell convergent extension heart nucleate erythrocyte intermediate cell mass of mesoderm posterior region nucleate erythrocyte pericardium
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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