Fig. 1

Neutrophils and macrophages have different morphologies and motility dynamics in live zebrafish. (A) Schematic diagram of a 3?dpf larva. The blue square corresponds to the imaging area for random leukocyte migration. The orange line corresponds to a tail transection wound to induce directed migration. See Movie 1. (B) Time-lapse images of a neutrophil (left) and a macrophage (right) randomly migrating. Bars are shown on the lower panel to indicate the distance migrated. The white cross marks the initial position of the cell body. Scale bar: 50?µm. (C?F) Macrophage and neutrophil perimeter (C), and roundness (D) were measured. 2D speed (E) and directionality (F) of neutrophils and macrophages was measured by manual tracking of time-lapse videos. Data are representative of three independent experiments. P-values were calculated with an unpaired two-tailed t-test.