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Fig. 1
Neutrophils and macrophages have different morphologies and motility dynamics in live zebrafish. (A) Schematic diagram of a 3 dpf larva. The blue square corresponds to the imaging area for random leukocyte migration. The orange line corresponds to a tail transection wound to induce directed migration. See Movie 1. (B) Time-lapse images of a neutrophil (left) and a macrophage (right) randomly migrating. Bars are shown on the lower panel to indicate the distance migrated. The white cross marks the initial position of the cell body. Scale bar: 50 µm. (C–F) Macrophage and neutrophil perimeter (C), and roundness (D) were measured. 2D speed (E) and directionality (F) of neutrophils and macrophages was measured by manual tracking of time-lapse videos. Data are representative of three independent experiments. P-values were calculated with an unpaired two-tailed t-test.