Fig. 3

Arp2/3 inhibition affects macrophage and neutrophil migration. (A) Time-lapse imaging of neutrophils and macrophages during incubation with CK-666 (200 µM). The inhibitor blocked neutrophil random motility, and strongly impaired macrophage random motility. Mean±s.d. velocity is shown; P-values were calculated with a least squares means analysis. (B) Inhibition of the Arp2/3 complex affects cell morphology. Time-lapse images showing a representative macrophage and neutrophil morphology. Arp2/3 inhibition induced neutrophil rounding (white arrowheads), whereas macrophages generated long and multiple filopodia-like protrusions (white arrows). Scale bar: 25 µm. (C) In response to tail transection, neutrophil recruitment was completely blocked by CK-666, but macrophage recruitment was only partly impaired. Data are mean±s.d. and are representative of three independent experiments; P-values were calculated with a two-tailed unpaired t-test.