FIGURE

Fig. S2

ID

ZDB-FIG-180122-24

Publication

Kim et al., 2017 - Claudin5a is required for proper inflation of Kupffer's vesicle lumen and organ laterality

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Fig. S2

Expression of Cldn5 in KV was ablated by two types of cldn5a translation-blocking MO.

(A—F) Maximum intensity projection images of Cldn5 (red) and sox17:egfp-positive KV cells (green) in 6 ss embryos. Representative images of standard control MO injected embryo (n = 9) (A), cldn5a translation-blocking MO¹ injected embryo (n = 11) (B), cldn5a translation-blocking MO² injected embryo (n = 11) (C), DFC-specific control morphants (n = 8) (D), DFC-specific cldn5a MO¹ injected embryo (n = 6) (E), and DFC-specific cldn5a MO² injected embryo (n = 7) (F). Scale bar: 20 μm.

Expression Data

Gene:	GFP
Antibody:	Ab1-cldn5
Fish:	s870Tg s870Tg + MO2-cldn5a s870Tg + MO3-cldn5a
Knockdown Reagents:	MO2-cldn5a MO3-cldn5a
Anatomical Term:	Kupffer's vesicle
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s870Tg + MO2-cldn5a s870Tg + MO3-cldn5a
Knockdown Reagents:	MO2-cldn5a MO3-cldn5a
Observed In:	Kupffer's vesicle
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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