PUBLICATION
Claudin5a is required for proper inflation of Kupffer's vesicle lumen and organ laterality
- Authors
- Kim, J.G., Bae, S.J., Lee, H.S., Park, J.H., Kim, K.W.
- ID
- ZDB-PUB-170805-14
- Date
- 2017
- Source
- PLoS One 12: e0182047 (Journal)
- Registered Authors
- Keywords
- Embryos, Cilia, Heart, Zebrafish, Epithelial cells, In situ hybridization, Organogenesis, Tight junctions
- MeSH Terms
-
- Animals
- Body Patterning/physiology*
- Cilia/physiology*
- Claudin-5/metabolism*
- Embryo, Nonmammalian/cytology*
- Embryo, Nonmammalian/metabolism
- Functional Laterality/physiology*
- Gene Expression Regulation, Developmental
- Morphogenesis/physiology*
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism*
- PubMed
- 28771527 Full text @ PLoS One
Citation
Kim, J.G., Bae, S.J., Lee, H.S., Park, J.H., Kim, K.W. (2017) Claudin5a is required for proper inflation of Kupffer's vesicle lumen and organ laterality. PLoS One. 12:e0182047.
Abstract
Left-right asymmetric organ development is critical to establish a proper body plan of vertebrates. In zebrafish, the Kupffer's vesicle (KV) is a fluid-filled sac which controls asymmetric organ development, and a properly inflated KV lumen by means of fluid influx is a prerequisite for the asymmetric signal transmission. However, little is known about the components that support the paracellular tightness between the KV luminal epithelial cells to sustain hydrostatic pressure during KV lumen expansion. Here, we identified that the claudin5a (cldn5a) is highly expressed at the apical surface of KV epithelial cells and tightly seals the KV lumen. Downregulation of cldn5a in zebrafish showed a failure in organ laterality that resulted from malformed KV. In addition, accelerated fluid influx into KV by combined treatment of forskolin and 3-isobutyl-1-methylxanthine failed to expand the partially-formed KV lumen in cldn5a morphants. However, malformed KV lumen and defective heart laterality in cldn5a morphants were significantly rescued by exogenous cldn5a mRNA, suggesting that the tightness between the luminal epithelial cells is important for KV lumen formation. Taken together, these findings suggest that cldn5a is required for KV lumen inflation and left-right asymmetric organ development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping