FIGURE

Fig. S1

ID

ZDB-FIG-180105-61

Publication

Li et al., 2017 - MiR-145 mediates zebrafish hepatic outgrowth through progranulin A signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

miR-145 mimic and miR-145 hairpin inhibitor treatment specificity and efficiently regulate miR-145 expression in vivo.

The miR-145 expression pattern at 72 hpf was examined using WISH and qPCR in control-, miR-145 inhibitor- and miR-145 mimic-injected fish. At 72 hpf, WISH indicates normal miR-145 expression in control-injected fish (A and B), reduced miR-145 expression in miR-145 inhibitor-injected fish (C and D) and overexpression of miR-145 in miR-145 mimic-injected fish (E and F). (A, C and E are dorsal views; B, D and F are lateral views). The qPCR analysis reveals that the miR-145 mimic and inhibitor modulate miR-145 expression (G). In addition, the qPCR analysis reveals that gata6 expression is regulated by altered expression of miR-145 (H). The ef1a and U6 expression was measured as a loading control. (**, P < 0.01, t-test; the arrow indicated liver, thirty embryos per experimental group were used and three independent replicates were performed).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One