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Fig. S1
miR-145 mimic and miR-145 hairpin inhibitor treatment specificity and efficiently regulate miR-145 expression in vivo.
The miR-145 expression pattern at 72 hpf was examined using WISH and qPCR in control-, miR-145 inhibitor- and miR-145 mimic-injected fish. At 72 hpf, WISH indicates normal miR-145 expression in control-injected fish (A and B), reduced miR-145 expression in miR-145 inhibitor-injected fish (C and D) and overexpression of miR-145 in miR-145 mimic-injected fish (E and F). (A, C and E are dorsal views; B, D and F are lateral views). The qPCR analysis reveals that the miR-145 mimic and inhibitor modulate miR-145 expression (G). In addition, the qPCR analysis reveals that gata6 expression is regulated by altered expression of miR-145 (H). The ef1a and U6 expression was measured as a loading control. (**, P < 0.01, t-test; the arrow indicated liver, thirty embryos per experimental group were used and three independent replicates were performed).