FIGURE

Fig. 6

ID
ZDB-FIG-180105-6
Publication
Yang et al., 2017 - Serotonin Activated Hepatic Stellate Cells Contribute to Sex Disparity in Hepatocellular Carcinoma
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Fig. 6

Manipulation of serotonin level and HSC density affects HSC activation and apoptosis. (A) IF staining and quantification of HSC density (Gfap+) in liver sections (n > 8 in each group). Three-month-old adult zebrafish were treated with dox with or without serotonin, BW23C86, PCPA, and SB204741 for 7 days. Percentages of activated HSCs (Gfap+/?-Sma+) in the liver sections (n > 8 in each group). (B) Expression of tgfb1 in hepatocytes, HSCs, neutrophils, macrophages, and cholangiocytes. These cells were isolated by fluorescence-activated cell sorting based on DsRed, GFP, DsRed, mCherry, and GFP expression, respectively, from fabp10+, hand2+, lyz+, mpeg+, and tp1+ transgenic zebrafish. Total RNAs were extracted and htr2b expression was determined by reverse-transcription quantitative PCR. Relative expression levels are with the values with female hepatocytes set as 1. (C and D) IF staining and quantification of percentages of (C) TgfB1+ HSCs (Gfap+/Tgfb1+) and (D) apoptotic HSCs in the liver sections as described in panel A. n > 8 in each group. *P < .05. Scale bar: 20 ?m.
Expression Data
Antibodies:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Mol Gastroenterol Hepatol
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