FIGURE

Fig. 5

ID

ZDB-FIG-180103-42

Publication

Yan et al., 2017 - RETRACTED: Chemical inhibition reveals differential requirements of signaling pathways in kras^V12- and Myc-induced liver tumors in transgenic zebrafish.

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Fig. 5

Cell apoptosis analysis of kras^V12- and Myc-induced carcinogenesis.

7 dpf WT, kras+ or Myc+ larvae were treated with 10 μM SU5402, 10 μM IWR1 or 10 μM cyclopamine in the presence of 10 μg/ml Dox. Apoptosis was analyzed by immunohistochemical staining with digoxigenin-conjugated nucleaotide and incubated with anti-digoxigenin secondary antibody. (A–D) Representative liver images of 7 dpf WT larvae. (E–H) Representative liver images of 7 dpf kras+ larvae. (I–L) Representative liver images of 7 dpf Myc+ larvae. (M) Statistical analysis of numbers of apoptotic cells in the liver of kras + larvae. (N) Statistical analysis of numbers of apoptotic cells in the liver for Myc+ larvae. N = 20 from each groups; statistical significance: *p < 0.05, Scale bar = 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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