ZFIN Image: Yan et al., 2017, Fig. 5

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Figure Caption

Fig. 5

Cell apoptosis analysis of kras^V12- and Myc-induced carcinogenesis.

7?dpf WT, kras+ or Myc+ larvae were treated with 10??M SU5402, 10??M IWR1 or 10??M cyclopamine in the presence of 10??g/ml Dox. Apoptosis was analyzed by immunohistochemical staining with digoxigenin-conjugated nucleaotide and incubated with anti-digoxigenin secondary antibody. (A?D) Representative liver images of 7?dpf WT larvae. (E?H) Representative liver images of 7?dpf kras+ larvae. (I?L) Representative liver images of 7?dpf Myc+ larvae. (M) Statistical analysis of numbers of apoptotic cells in the liver of kras?+?larvae. (N) Statistical analysis of numbers of apoptotic cells in the liver for Myc+ larvae. N?=?20 from each groups; statistical significance: *p?<?0.05, Scale bar?=?20??m.

Acknowledgments

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