FIGURE

Fig. 2

ID

ZDB-FIG-171206-84

Publication

Wan et al., 2017 - Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity

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Fig. 2

Cellular and molecular identification of zebrafish γδ T cells. (A) RT-PCR assay analyzed the expression of T cell markers (γ, δ, α, β, CD4, and CD8), antigen-presenting cell marker (MHC-II), B cell marker (mIgM), and myeloid cell (monocyte/macrophage and dendritic cell) markers (CSF-1R and FcεRI). The expressions of these markers from negative selection cells were used as control. (B) FCM analyzed the FSC/SSC profiles of leukocytes and the sorted cells. (C) Transmission electron microscopy and scanning electron microscopy show the detailed morphologies of sorted γδ T cells. Scale bar 1 µm (bottom left). (D) Wright-Giemsa staining indicates the morphologies of the sorted γδ T cells. Scale bar 10 µm (bottom left). Original magnification ×1,000. (E) Detect the expressions of γ and δ in the anti-γ and anti-δ pull down cells, respectively, by Western blot.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol