Fig. 4

Phagocytic ability of zebrafish γδ T cells. (A) FCM detected the phagocytic ability. γδ T cells were magnetically sorted and incubated with FITC-KLH, 1 µm red fluorescent latex beads or FITC-A.h at 28°C for 4 h. Cells in control group for active phagocytosis were incubated in ice. In parallel, γδ T cells incubated with FITC-KLH, red fluorescent beads, and FITC-A.h (28°C for 4 h) in the presence of cytochalasin B were set as controls. The numbers above the marker bars in each panel indicate the percentages of phagocytic γδ T cells. The geometric means of the fluorescence intensities computed from the outlined region represent the phagocytic ability of γδ T cells in each treatment group. Means ± SD of three independent experiments are shown. *P < 0.05, **P < 0.01. (B) Confocal microscopy of 1 µm red fluorescent latex beads by γδ T cells. DAPI stain showed the location of the nuclei. Original magnification ×630. Scale bar, 5 µm.