|
Fig. 2
Cellular and molecular identification of zebrafish γδ T cells. (A) RT-PCR assay analyzed the expression of T cell markers (γ, δ, α, β, CD4, and CD8), antigen-presenting cell marker (MHC-II), B cell marker (mIgM), and myeloid cell (monocyte/macrophage and dendritic cell) markers (CSF-1R and FcεRI). The expressions of these markers from negative selection cells were used as control. (B) FCM analyzed the FSC/SSC profiles of leukocytes and the sorted cells. (C) Transmission electron microscopy and scanning electron microscopy show the detailed morphologies of sorted γδ T cells. Scale bar 1 µm (bottom left). (D) Wright-Giemsa staining indicates the morphologies of the sorted γδ T cells. Scale bar 10 µm (bottom left). Original magnification ×1,000. (E) Detect the expressions of γ and δ in the anti-γ and anti-δ pull down cells, respectively, by Western blot.