FIGURE

Fig. 3

ID

ZDB-FIG-171206-85

Publication

Wan et al., 2017 - Characterization of γδ T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity

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Fig. 3

Figure 3. Immunophenotype analysis of zebrafish γδ T cells by Abs against various surface molecules. The magnetic sorted cells were double stained with anti-γ, anti-δ, anti-α, anti-β, anti-CD8, and anti-CD4 Abs in different combinations (rabbit anti-γ/mouse anti-δ, rabbit anti-β/mouse anti-α, rabbit anti-γ/mouse anti-α, rabbit anti-β/mouse anti-δ, rabbit anti-CD8/mouse anti-δ, rabbit anti-CD4/mouse anti-δ, and rabbit anti-CD4/mouse anti-α, respectively). Positive control cells were stained with rabbit anti-β and mouse anti-α Abs. Non-related Abs, including mouse IgG and rabbit IgG, were used as negative controls (data not shown). DAPI stain showed the locations of the nuclei. A laser-scanning confocal microscopy (Zeiss LSM-710) was used in the analyses (original magnification × 630, scale bar, 5 µm).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol