FIGURE

Fig. S10

ID

ZDB-FIG-171201-39

Publication

Khatri et al., 2016 - Down-regulation of coasy, the gene associated with NBIA-VI, reduces Bmp signaling, perturbs dorso-ventral patterning and alters neuronal development in zebrafish

Other Figures

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Fig. S10

Acridine Orange staining.

A) Representative images of embryos not-injected (A, B) and injected with 1.2 pmol/embryo of ST-MO (C, D) or coasy-MO (E, F) at 48 hpf obtained by acridine orange staining to assess the presence of dead cells. Morphants show a significant increase of the fluorescence staining, particularly in brain and tail. B) The quantification of the fluorescence intensity performed by a plate reader shows a significant (P<0.001), 2.5-fold increase in coasy-MO-injected embryos. Results are from a representative experiment with at least 50 embryos, repeated at least three times.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-coasy
Knockdown Reagent:	MO1-coasy
Observed In:	brain cell death post-vent region cell death
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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