FIGURE

Fig. 3

ID

ZDB-FIG-171115-3

Publication

Lien et al., 2016 - Zebrafish cyclin Dx is required for development of motor neuron progenitors, and its expression is regulated by hypoxia-inducible factor 2α

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Fig. 3

The role of zccndx in cell proliferation and regulation of the numbers of motor neuron progenitors (pMNs).

(A) Confocal images showing lateral views of the heads of control-MO-injected (panel a) or ccndx-MO-injected (panel b) embryos stained with anti-pH3 (red in panels a and b) or anti-BrdU (green in d and e). Panels c and f: graphs showing the number of proliferating cells (pH3 or BrdU positive) at 24 hpf. The asterisks indicate significant differences between the control and experimental morphants. The n value is indicated. (B) Whole–mount in situ hybridization of motor neurons and interneuron markers in control-MO and zccndx ATG-MO-injected embryos. Probes against olig2 (a and b), nkx6.1(c and d), isl1 (e and f), and vsx1 (g and h) were used for detection. control MO-injected embryos are shown in panels a, c, e and g while ccndx ATG-MO-injected embryos are shown in panels b, d, f and h. Motor neurons (MN) were lost (panels b, f, lost MNs indicated by arrowheads), while interneurons (IN) (panels d and h) were unaffected. Scale bar, 100 mm.

Expression Data

Genes:	isl1a nkx6.1 olig2 vsx1
Fish:	WT + MO1-ccndx
Knockdown Reagent:	MO1-ccndx
Anatomical Terms:	interneuron motor neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ccndx
Knockdown Reagent:	MO1-ccndx
Observed In:	brain cell population proliferation interneuron motor neuron primary motor neuron
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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