FIGURE

Fig. 3

ID

ZDB-FIG-171108-13

Publication

Cai et al., 2016 - JNK Inhibition Inhibits Lateral Line Neuromast Hair Cell Development

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Fig. 3

Figure 3. Effects of SP600125 on apoptosis. (A–B) Cleaved caspase-3 staining in the neuromast from a 5 dpf control larva (A) and 15 μM SP600125-treated 5 dpf larva (B). Scale bar = 10 μm. (C) SP600125 treatment increased the numbers of cleaved caspase−3−positive cells and cleaved caspase−3−positive hair cells [one-way ANOVA; Caspase−3+ cells: F_{(2, 101)} = 12.53, p < 0.001; Caspase3+ hair cells: F_{(2, 101)} = 4.549, p = 0.0128]. Bars are mean ± SD. n = 24 neuromasts in control, n = 24 in 10 μM SP600125-treated neuromasts, and n = 56 in 15 μM SP600125-treated neuromasts. *p < 0.05, significant when compared to control larvae; **p < 0.01, highly significant when compared to control larvae; ***p < 0.001, highly significant when compared to control larvae. (D) After treatment of 3 dpf larvae with 15 μM SP600125 for 2 days, protein extracts were prepared and subjected to western blot assay using an antibody against cleaved caspase-3. β-Actin was included as the loading control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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