|
Fig. 3
Figure 3. Effects of SP600125 on apoptosis. (A–B) Cleaved caspase-3 staining in the neuromast from a 5 dpf control larva (A) and 15 μM SP600125-treated 5 dpf larva (B). Scale bar = 10 μm. (C) SP600125 treatment increased the numbers of cleaved caspase−3−positive cells and cleaved caspase−3−positive hair cells [one-way ANOVA; Caspase−3+ cells: F(2, 101) = 12.53, p < 0.001; Caspase3+ hair cells: F(2, 101) = 4.549, p = 0.0128]. Bars are mean ± SD. n = 24 neuromasts in control, n = 24 in 10 μM SP600125-treated neuromasts, and n = 56 in 15 μM SP600125-treated neuromasts. *p < 0.05, significant when compared to control larvae; **p < 0.01, highly significant when compared to control larvae; ***p < 0.001, highly significant when compared to control larvae. (D) After treatment of 3 dpf larvae with 15 μM SP600125 for 2 days, protein extracts were prepared and subjected to western blot assay using an antibody against cleaved caspase-3. β-Actin was included as the loading control.