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ZFIN ID: ZDB-FIG-171027-22
Novodvorsky et al., 2015 - klf2ash317 Mutant Zebrafish Do Not Recapitulate Morpholino-Induced Vascular and Haematopoietic Phenotypes. PLoS One   10:e0141611 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-25

Fig. S5

Expression of HSC markers runx1 and cmyb is blood flow dependent.

Expression patterns of HSC markers runx1 (top panel) and cmyb (bottom panel) is significantly diminished in the tnnt2 morphants which lack blood flow confirming previously published data [19]. Black arrows indicate the aorta-gonad-mesonephros (AGM) region and red arrows indicate caudal haematopoietic tissue (CHT). Numbers in the top left corners indicate number of embryos with similar staining patterns out of total number of embryos examined. Scale bar = 500μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myb WT standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO1-tnnt2a standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
runx1 WT standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO1-tnnt2a standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tnnt2a standard conditions Prim-25 ventral wall of dorsal aorta hematopoietic stem cell myb expression decreased amount, abnormal
Prim-25 ventral wall of dorsal aorta hematopoietic stem cell runx1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One