header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-171027-19
Novodvorsky et al., 2015 - klf2ash317 Mutant Zebrafish Do Not Recapitulate Morpholino-Induced Vascular and Haematopoietic Phenotypes. PLoS One   10:e0141611 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. S1

klf2a vascular expression is blood flow-dependent.

klf2a is expressed in zebrafish embryonic vasculature of a WT embryo at 48hpf. Cessation of blood flow in the trunk vasculature by an occlusion of proximal aorta in the gridlock mutants (indicated by a green arrow) results in a complete loss of klf2a vascular expression distally to the occlusion. Blockage of embryonic heart contractions by tnnt2 MO results in significantly decreased klf2a vascular expression. Pharmacological inhibition of heart contractions by tricaine from 32 to 48hpf results in significantly decreased klf2a vascular expression. Interestingly tricaine also reduces klf2a expression in the heart region and in the cells lateral to the most posterior notochord. Red arrows indicate trunk vasculature, black arrows indicate the cells lateral to most posterior notochord, white arrows indicate pectoral fins, black arrowheads indicate the cardiac outflow tract and white arrowheads indicate cloaca. Numbers in top right corners indicate the number of embryos with similar staining pattern out of all embryos examined. klf2a riboprobe used. Scale bar = 500μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
klf2a WT control Long-pec heart ISH
Long-pec trunk vasculature ISH
WT chemical treatment by environment: tricaine Long-pec heart ISH
Long-pec trunk vasculature ISH
WT + MO1-tnnt2a standard conditions Long-pec trunk vasculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: tricaine Long-pec heart klf2a expression decreased amount, abnormal
Long-pec trunk vasculature klf2a expression decreased amount, abnormal
WT + MO1-tnnt2a standard conditions Long-pec trunk vasculature klf2a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One