FIGURE

Fig. 4

ID

ZDB-FIG-171025-16

Publication

Liu et al., 2015 - Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish

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Fig. 4

Comparison of EGFP expression patterns in three ET lines with those of corresponding endogenous genes.

(A, B, C and D) Wide-type (WT) and pTME1 embryos were collected at 3 dpf and WISH assays were performed with antisense RNA probes for tcf7l2 or egfp, respectively. (E and F) WT and pTME3 embryos were fixed at 24 hpf for WISH assays. WT embryos were detected with probe hs3st3b1b (E) and pTME3 embryos with probe egfp (F). (G, H, I and J) WISH assays for WT or pTME9 embryos at 3 dpf were conducted with antisense RNA probes for mecom or egfp. Embryos were viewed in lateral (A, B, E, F, G and H) and dorsal (C, D, I and J).

Expression Data

Genes:	EGFP hs3st3b1b mecom tcf7l2
Fish:	AB ihb205Et (AB) ihb206Et (AB) zf1019Et (AB)
Anatomical Terms:	brain cranial ganglion diencephalon dorsal fin gut hindbrain lateral line primordium midbrain pectoral fin pectoral fin bud pharyngeal arch 3-7 proctodeum rhombomere spinal cord swim bladder tegmentum
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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