Fig. S4

Klf6a mediated HSPCs expansion is specific. Related to Figure 4.

(A) The statistical data of the number of coro1a:GFP donor cells derived from wild-type kidney marrow in sibling recipients and klf6a^-/- recipients at different post-transplant time points (mean ± SD, t-test, **P<0.01, ns>0.05). (B) The statistical result of the cell number of sibling and klf6a^-/- derived 48 hpf coro1a:GFP cells in wild-type recipients at different post-transplant time points (mean ± SD, t-test, ns>0.05). (C) Confocal imaging of parabiotic pairs at 60 hpf. The control embryos were CD41:GFP background and the klf6a morphants were sclα:dsRed background. This image was merged by two pictures of different views. Scale bar, 100 μm. (D) The magnified images of CHT are shown. The HSPCs are indicated by white arrowheads. Scale bar, 100 μm. (E) The schematic diagram showing the construct of truncated Klf6a driven by the fli1 promoter. GFP indicated the expression of truncated protein in fli1⁺ cells. (F) Expression of klf6a, cmyb and ae1- globin in overexpressed truncated Klf6a (GFP⁺) embryos or control embryos (GFP^-), respectively, at 36 hpf or 3 dpf. (G) The schematic diagram showing the construct of full length Klf6a driven by the fli1 promoter. GFP indicated the expression of Klf6a protein in fli1⁺ cells. (H) qPCR result of cmyb, pu.1 and ae1-globin expression in control, klf6a morphants and embryos co-injected with fli1-Klf6GFP and klf6a MOs (mean ± SD, t-test, **P<0.01,***P<0.001, n = 3). (I) WISH data of cmyb, lyz and ae1-globin in these three groups.