|ZFIN ID: ZDB-FIG-170921-90|
Knockdown of sept7b reduces α-cell volume and increases bihormonal cells positive for both insulin and glucagon.
(A,B) Isosurface rendered z-stack confocal images generated from control MO-injected (A) and sept7b TBMO-injected (B) Tg(gcg:GFP) zebrafish larva at 4 dpf. Glucagon-positive α-cells are green. (C) Glucagon-positive cell volume is significantly reduced in sept7b TBMO-injected zebrafish larvae at 4 dpf. (D,E) Cells positive for both insulin (red) and glucagon (green) (bihormonal cells; arrowheads) are increased in sept7b TBMO-injected Tg(gcg:GFP) zebrafish larvae 4 dpf (E) compared to control MO-injected larva (D). In (D,E), nuclei are labeled with DAPI (blue). (F) Quantification of the percentage of bihormonal cells relative to insulin positive cells. Scale bar: (A,B) (25 μm); (D,E) (20 μm). Error bars represent mean ± SEM. ***p ≤ 0.0005.
|GFP||ia1Tg||standard conditions||Day 4||IHC|
|ia1Tg + MO1-sept15||standard conditions||Day 4||IHC|
|Ab1-ins||ia1Tg + MO1-sept15||standard conditions||Day 4||IHC|
|ia1Tg + MO1-sept15||standard conditions||Day 4||endocrine pancreas has fewer parts of type pancreatic A cell, abnormal|
|Day 4||pancreas pancreatic A cell differentiation decreased occurrence, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.|