ZFIN ID: ZDB-FIG-170921-90
Dash et al., 2016 - sept7b is required for the differentiation of pancreatic endocrine progenitors. Scientific Reports   6:24992 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 3

Knockdown of sept7b reduces α-cell volume and increases bihormonal cells positive for both insulin and glucagon.

(A,B) Isosurface rendered z-stack confocal images generated from control MO-injected (A) and sept7b TBMO-injected (B) Tg(gcg:GFP) zebrafish larva at 4 dpf. Glucagon-positive α-cells are green. (C) Glucagon-positive cell volume is significantly reduced in sept7b TBMO-injected zebrafish larvae at 4 dpf. (D,E) Cells positive for both insulin (red) and glucagon (green) (bihormonal cells; arrowheads) are increased in sept7b TBMO-injected Tg(gcg:GFP) zebrafish larvae 4 dpf (E) compared to control MO-injected larva (D). In (D,E), nuclei are labeled with DAPI (blue). (F) Quantification of the percentage of bihormonal cells relative to insulin positive cells. Scale bar: (A,B) (25 μm); (D,E) (20 μm). Error bars represent mean ± SEM. ***p ≤ 0.0005.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ia1Tg standard conditions Day 4 pancreatic A cell cytoplasm IHC
ia1Tg + MO1-sept15 standard conditions Day 4 pancreatic A cell cytoplasm IHC
ins Ab1-ins ia1Tg control Day 4 pancreatic A cell cytoplasm IHC
Ab1-ins Day 4 pancreatic B cell cytoplasm IHC
Ab1-ins ia1Tg + MO1-sept15 standard conditions Day 4 pancreatic A cell cytoplasm IHC
Ab1-ins Day 4 pancreatic B cell cytoplasm IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ia1Tg + MO1-sept15 standard conditions Day 4 endocrine pancreas has fewer parts of type pancreatic A cell, abnormal
Day 4 pancreas pancreatic A cell differentiation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.