FIGURE

Fig. 5

ID

ZDB-FIG-170921-31

Publication

Bergen et al., 2017 - The Golgi matrix protein giantin is required for normal cilia function in zebrafish

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Fig. 5

Left-right asymmetry and phenotypic rescue of giantin MO. (A) Cardiomyocyte Cmcl2::GFP transgenic reporter line outlining the heart position (arrows, blue: left; green: midline; white: right) at 32 hpf. (B) Representative confocal image of 48 hpf with ATG morpholino and Rn-Golgb1 mRNA showing Golgi localisation of Rn-Golgb1 in dermal cells stained for anti-flag (white arrows). White dotted line indicates cell boundaries. N=3 individuals. (C) Quantification of heart position (32 hpf), additionally showing Rn-Golgb1 mRNA co-injection rescue. (D) Stereomicroscope images of 3 dpf control and E14 morpholinos injected with same dose in WT and golgb1^{Q2948X/Q2948X} individuals derived from same day WTxWT and golgb1^{Q2948X/Q2948X} x golgb1^{Q2948X/Q2948X} in-crosses with (E) quantification of phenotypes scored. (F) Quantitative RT-PCR of golgins showing relative mRNA expression levels on 5 dpf larvae. Normalised to beta-actin, mean and standard deviation. All data from three independent experiments.

Expression Data

Genes:	EGFP gapdh gcc1 golga1 golga2 golga3 golga4 golga5 golgb1 gorab gorasp1a gorasp2 hprt1 man2a1 tmf1 trip11 uso1
Fish:	WT golgb1^{sa11389/sa11389} twu34Tg twu34Tg + MO1-golgb1 twu34Tg + MO2-golgb1
Knockdown Reagents:	MO1-golgb1 MO2-golgb1
Anatomical Terms:	heart tube whole organism
Stage Range:	Prim-15 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	golgb1^{sa11389/sa11389} WT + MO2-golgb1 twu34Tg + MO1-golgb1 twu34Tg + MO2-golgb1
Knockdown Reagents:	MO1-golgb1 MO2-golgb1
Observed In:	determination of heart left/right asymmetry eye heart heart tube whole organism
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

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