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ZFIN ID: ZDB-FIG-170921-31
Bergen et al., 2017 - The Golgi matrix protein giantin is required for normal cilia function in zebrafish. Biology Open   6(8):1180-1189 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 5

Left-right asymmetry and phenotypic rescue of giantin MO. (A) Cardiomyocyte Cmcl2::GFP transgenic reporter line outlining the heart position (arrows, blue: left; green: midline; white: right) at 32 hpf. (B) Representative confocal image of 48 hpf with ATG morpholino and Rn-Golgb1 mRNA showing Golgi localisation of Rn-Golgb1 in dermal cells stained for anti-flag (white arrows). White dotted line indicates cell boundaries. N=3 individuals. (C) Quantification of heart position (32 hpf), additionally showing Rn-Golgb1 mRNA co-injection rescue. (D) Stereomicroscope images of 3 dpf control and E14 morpholinos injected with same dose in WT and golgb1Q2948X/Q2948X individuals derived from same day WTxWT and golgb1Q2948X/Q2948X x golgb1Q2948X/Q2948X in-crosses with (E) quantification of phenotypes scored. (F) Quantitative RT-PCR of golgins showing relative mRNA expression levels on 5 dpf larvae. Normalised to beta-actin, mean and standard deviation. All data from three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Prim-15 heart tube IFL
twu34Tg + MO1-golgb1 standard conditions Prim-15 heart tube IFL
twu34Tg + MO2-golgb1 standard conditions Prim-15 heart tube IFL
gapdh WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
gcc1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
golga1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
golga2 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
golga3 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
golga4 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
golga5 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
golgb1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
gorab WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
gorasp1a WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
gorasp2 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
hprt1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
man2a1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
tmf1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
trip11 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
uso1 WT standard conditions Day 5 whole organism RTPCR
golgb1sa11389/sa11389 standard conditions Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-golgb1 standard conditions Protruding-mouth eye decreased size, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth whole organism decreased length, abnormal
golgb1sa11389/sa11389 control Protruding-mouth whole organism morphology, normal
golgb1sa11389/sa11389 + MO2-golgb1 standard conditions Protruding-mouth eye size, ameliorated
Protruding-mouth whole organism length, ameliorated
twu34Tg + MO1-golgb1 standard conditions Prim-15 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-15 heart tube mislocalised, abnormal
twu34Tg + MO2-golgb1 standard conditions Prim-15 determination of heart left/right asymmetry decreased process quality, abnormal
Prim-15 heart tube mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open