FIGURE

Fig. S1

ID

ZDB-FIG-170824-49

Publication

Zhang et al., 2016 - Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors

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Fig. S1

Expression of GCaMPHS in BCs and electrophysiological properties of BCs in zebrafish larvae.

(a) Confocal image showing GCaMPHS expression in BCs of a 3-dpf Tg(Gal4-VP16^xfz43,UAS:GCaMPHS) larva.

(b) Enlarged view of the boxed region in (a), showing the GCaMPHS expression in the soma, axon and AT of BCs. In vivo whole-cell recording was performed on BC somata.

(c) Light-evoked responses of an OFF subtype BC in response to a 2-s flash (bottom) under current- (top) or voltage-clamp mode (middle; holding potential, -60 mV).

(d) Summary of data showing the resting membrane potentials (V_m) of BCs at four different retinal regions. The number in the brackets indicates the number of BCs examined. T, D, N and V indicate the temporal, dorsal, nasal and ventral retina, respectively.

One-way ANOVA for the data in (d). Data are represented as mean ± s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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