ZFIN Image: Zhang et al., 2016, Fig. S1

Image

Figure Caption

Fig. S1

Expression of GCaMPHS in BCs and electrophysiological properties of BCs in zebrafish larvae.

(a) Confocal image showing GCaMPHS expression in BCs of a 3-dpf Tg(Gal4-VP16^xfz43,UAS:GCaMPHS) larva.

(b) Enlarged view of the boxed region in (a), showing the GCaMPHS expression in the soma, axon and AT of BCs. In vivo whole-cell recording was performed on BC somata.

(c) Light-evoked responses of an OFF subtype BC in response to a 2-s flash (bottom) under current- (top) or voltage-clamp mode (middle; holding potential, -60 mV).

(d) Summary of data showing the resting membrane potentials (V_m) of BCs at four different retinal regions. The number in the brackets indicates the number of BCs examined. T, D, N and V indicate the temporal, dorsal, nasal and ventral retina, respectively.

One-way ANOVA for the data in (d). Data are represented as mean ± s.e.m.

Acknowledgments

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