PUBLICATION

Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors

Authors
Zhang, R.W., Li, X.Q., Kawakami, K., Du, J.L.
ID
ZDB-PUB-160903-8
Date
2016
Source
Nature communications   7: 12650 (Journal)
Registered Authors
Du, Jiu Lin, Kawakami, Koichi
Keywords
Neuronal development, Retina
MeSH Terms
  • Presynaptic Terminals/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Electrical Synapses/physiology
  • Retinal Ganglion Cells/metabolism*
  • Receptors, N-Methyl-D-Aspartate/metabolism*
  • Larva/growth & development
  • Synaptic Transmission/physiology*
  • Retinal Bipolar Cells/metabolism*
  • Glutamic Acid/metabolism
  • Visual Pathways/physiology*
  • Animals
(all 12)
PubMed
27586999 Full text @ Nat. Commun.
Abstract
Glutamatergic retinal waves, the spontaneous patterned neural activities propagating among developing retinal ganglion cells (RGCs), instruct the activity-dependent refinement of visuotopic maps. However, its initiation and underlying mechanism remain largely elusive. Here using larval zebrafish and multiple in vivo approaches, we discover that bipolar cells (BCs) are responsible for the generation of glutamatergic retinal waves. The wave originates from BC axon terminals (ATs) and propagates laterally to nearby BCs and vertically to downstream RGCs and the optic tectum. Its initiation is triggered by the activation of and consequent glutamate release from BC ATs, and is mediated by the N-methyl-D-aspartate subtype of glutamate receptors (NMDARs) expressed at these ATs. Intercellular asymmetry of NMDAR expression at BC ATs enables the preferential initiation of waves at the temporal retina, where BC ATs express more NMDARs. Thus, our findings indicate that glutamatergic retinal waves are initiated by BCs through a presynaptic NMDA autoreceptor-dependent process.
Genes / Markers
Figures
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    ion13dTgTransgenic Insertion
      ion14dTgTransgenic Insertion
        ion17dTgTransgenic Insertion
          nkGCaMPHS4aTgTransgenic Insertion
            s1992tTgTransgenic Insertion
              s1993tTgTransgenic Insertion
                ub43TgTransgenic Insertion
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  No data available
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  CFPEFGCFP
                  ChR2EFGChR2
                  cpEGFPEFGcpEGFP
                  EGFPEFGEGFP
                  GAL4EFGGAL4
                  GCaMPEFGGCaMP
                  mCherryEFGmCherry
                  NTREFGNTR
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                  Mapping
                  No data available
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                  Citation
                  Zhang, R.W., Li, X.Q., Kawakami, K., Du, J.L. (2016) Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors. Nature communications. 7:12650.