ZFIN ID: ZDB-FIG-170815-30
Mazon-Moya et al., 2017 - Septins restrict inflammation and protect zebrafish larvae from Shigella infection. PLoS pathogens   13:e1006467 Full text @ PLoS Pathog.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3

S. flexneri induces neutrophil death in Sept15 morphants.

A-B. Quantification of neutrophils in lyz:dsRed larvae injected with (A) Ctrl or (B) Sept15 morpholino (Mo), uninfected (open circles) or infected for 6 h with a low (closed circles) or high (grey circles) dose of S. flexneri M90T, from 4 or more larvae per treatment from 3 independent experiments. Circles represent individual larvae. Mean ± SEM also shown (horizontal bars). Significance testing performed by ANOVA with Bonferroni posttest. **, P<0.01; ***, P<0.001. C. Representative images of lyz:dsRed larvae injected with Ctrl, Sept15, or Sept15 + Irf8 Mo. Scale bar, 250 μm. D. Quantification of neutrophils in lyz:dsRed larvae injected with Ctrl (open circles), Sept15 (closed circles), or Sept15 + Irf8 (grey circles) Mo. Circles represent individual larvae. Mean ± SEM also shown (horizontal bars). Significance testing performed by ANOVA with Bonferroni posttest. ***, P<0.001. E. Survival curves of Sept15 or Sept15 + Irf8 morphants infected with S. flexneri M90T (low dose). Pooled data from 3 independent experiments per treatment using at least 15 larvae per treatment. Significance testing performed by Log Rank test. F. Enumeration of bacteria at 0, 24, or 48 hpi from Sept15 (open circles) or Sept15 + Irf8 (closed circles) morphants infected with S. flexneri M90T (low dose). Half-filled circles represent enumerations from larvae at time 0 and are representative of inocula for both conditions. Pooled data from 3 independent experiments per inoculum class using up to 3 larvae per treatment. Circles represent individual larvae, and only larvae having survived the infection (thus far) included here (i.e., dead larvae not homogenised for counts). Mean ± SEM also shown (horizontal bars). Significance testing performed by Student’s t test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 nz50Tg bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil IFL
nz50Tg + MO1-irf8 + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil IFL
nz50Tg + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz50Tg bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil decreased amount, abnormal
nz50Tg + MO1-irf8 + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil decreased amount, ameliorated
Protruding-mouth whole organism decreased life span, abnormal
nz50Tg + MO3-sept15 control Protruding-mouth neutrophil decreased amount, abnormal
nz50Tg + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil decreased amount, exacerbated
Protruding-mouth whole organism decreased life span, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS pathogens for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Pathog.