ZFIN ID: ZDB-FIG-170815-31
Mazon-Moya et al., 2017 - Septins restrict inflammation and protect zebrafish larvae from Shigella infection. PLoS pathogens   13:e1006467 Full text @ PLoS Pathog.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 4

Sept15 restricts the inflammatory response in vivo.

A. Representative frames extracted from in vivo time-lapse confocal imaging of Tg(il-1b:GFP-F) x Tg(lyz:dsRed) larvae (3 dpf) injected with Ctrl or Sept15 Mo and infected in the HBV with Crimson-S. flexneri M90T (low dose) at 19 hpi. Maximum intensity Z-projection images (2 μm serial optical sections) are shown. Scale bars, 50 μm. See also S8 and S9 Videos. B. Relative % of Caspase-1 activity levels measured in Ctrl and Sept15 morphants infected for 6 h with ~5 x 103 CFU of S. flexneri M90T. Mean±SEM from 3 independent experiments per treatment using 5–10 larvae per experimental group. Significance testing performed by Student’s t test. **; P<0.01. C. Representative images of Ctrl and Sept15 morphants (Mo) infected with S. flexneri M90T in the HBV and stained for acridine orange. Dotted line shows the area where cells were quantified (i.e. the infected HBV). Scale bars, 100 μm. E, eye. D. Number of acridine orange (AO) positive cells counted in the HBV of Ctrl or Sept15 morphants 6 hpi with S. flexneri M90T. Significance testing performed by Student’s t test. **, P<0.001. Pooled data from 3 independent experiments per treatment using at least 6 larvae per treatment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 nz50Tg; zf550Tg bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil IFL
nz50Tg; zf550Tg + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth neutrophil IFL
GFP nz50Tg; zf550Tg bacterial treatment by injection: Shigella flexneri Protruding-mouth epithelial cell IFL
Protruding-mouth macrophage IFL
Protruding-mouth neutrophil IFL
nz50Tg; zf550Tg + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth epithelial cell IFL
Protruding-mouth macrophage IFL
Protruding-mouth neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth cell death increased occurrence, abnormal
nz50Tg; zf550Tg + MO3-sept15 bacterial treatment by injection: Shigella flexneri Protruding-mouth cell death increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS pathogens for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Pathog.