FIGURE

Fig. 12

ID

ZDB-FIG-170807-42

Publication

Murphy et al., 2017 - Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish

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Fig. 12

Suppression of cell death has little effect on disrupted eng2a expression in brd2aMO morphants.

TUNEL assay and in situ hybridization to eng2a RNA in brd2aMO morphants in which cell death is suppressed by A–L) treatment with pan-caspase inhibitor Q-VD-OPh; or M–X) treatment with p53MO co-injection. For pan-caspase study: wildtype DMSO (A,B,C), Q-VD-OPh (D,E,F), brd2aMO (G,H,I), and brd2aMO + Q-VD-OPh (J,K,L) treated embryos were assessed for apoptotic nuclei by TUNEL assay followed by laser scanning confocal microscopy (brightfield A,D,G,J; darkfield B,E,H,K); and for eng2a expression by in situ hybridization (C,F,I,L). brd2aMO morphants exhibit reduced/absent eng2a expression in the anterior hindbrain domain compared to controls, regardless of the suppression of cell death by pan-caspase inhibition (arrows in I and L versus C and F). Refer to E for quantitative analysis.

Expression Data

Gene:	en2a
Fish:	AB AB + MO3-brd2a AB + MO3-brd2a + MO4-tp53 AB + MO4-tp53
Condition:	chemical treatment: apoptosis inhibitor
Knockdown Reagents:	MO3-brd2a MO4-tp53
Anatomical Terms:	midbrain hindbrain boundary midbrain hindbrain boundary posterior region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-brd2a AB + MO3-brd2a + MO4-tp53
Condition:	chemical treatment: apoptosis inhibitor
Knockdown Reagents:	MO3-brd2a MO4-tp53
Observed In:	brain apoptotic process midbrain hindbrain boundary posterior region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.