FIGURE

Fig. 2

ID

ZDB-FIG-170807-34

Publication

Murphy et al., 2017 - Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish

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Fig. 2

Morphological defects of brd2aMO morphants are substantially rescued by co-injection of human Brd2 mRNA.

Morpholino injections were conducted with and without co-injection of in vitro transcribed human Brd2 RNA using a total of at least three clutches and 40 embryos per treatment. Lateral views of the head region of representative prim 5 stage embryos show brain structure in A) uninjected wildtype, B) embryo injected with 250 pg in vitro synthesized human Brd2 mRNA, C) embryo injected with 4 ng brd2aMO2, and D) embryo co-injected with 4 ng brd2aMO2 and 250 pg human Brd2 mRNA. Co-injection with Brd2 mRNA reduces the severity of brain and trunk defects, often restoring the size and structure of the MHB region in a good fraction of brd2aMO2 morphant embryos (D, arrow).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO4-brd2a
Knockdown Reagent:	MO4-brd2a
Observed In:	brain fourth ventricle midbrain hindbrain boundary
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 146, Murphy, T., Melville, H., Fradkin, E., Bistany, G., Branigan, G., Olsen, K., Comstock, C.R., Hanby, H., Garbade, E., DiBenedetto, A.J., Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary (MHB) region in zebrafish, 10-30, Copyright (2017) with permission from Elsevier. Full text @ Mech. Dev.