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ZFIN ID: ZDB-FIG-170807-3
Wu et al., 2017 - tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae.. Gene Expression   17(3):237-249 Full text @ Gene Expr.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 2

Cell death and proliferation in tomm22 MO-injected larvae. (A) Confocal projection images showing Prox1 expression (gray) and terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) (green) in the liver bud (dashed lines) at 36 h postfertilization (hpf). Arrow points to TUNEL+ cells. (B) Confocal projection images showing TUNEL (green) and the expression of fabp10a:CFP-NTR (blue) and Tp1:H2B-mCherry (red) in the liver at 4 dpf. Arrow points to TUNEL+ hepatocytes. (C) Confocal projection images showing Prox1 expression (green) and 5-ethynyl-2′-deoxyuridine (EdU) labeling (gray) in the liver (dashed lines) at 45 hpf. Arrows point to EdU/Prox1 double-positive cells. (D) Graph showing the percentage of EdU+ cells among Prox1+ cells in the liver at 45 hpf. There was no significant difference in the proliferation rate between the control and tomm22 MO-injected larvae. Red marks indicate the embryos shown in (C); n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
CFP s931Tg ; s939Tg control Day 4 hepatocyte IHC
s931Tg ; s939Tg + MO1-tomm22 standard conditions Day 4 hepatocyte IHC
mCherry s931Tg ; s939Tg control Day 4 cholangiocyte IHC
s931Tg ; s939Tg + MO1-tomm22 standard conditions Day 4 cholangiocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tomm22 standard conditions Prim-25 liver cell death increased occurrence, abnormal
High-pec liver cell death increased occurrence, abnormal
s931Tg ; s939Tg + MO1-tomm22 standard conditions Day 4 liver cell death increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Gene Expression for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Gene Expr.