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ZFIN ID: ZDB-FIG-170807-4
Wu et al., 2017 - tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae.. Gene Expression   17(3):237-249 Full text @ Gene Expr.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 3

Biliary epithelial cells (BECs) give rise to hepatocytes in tomm22 MO-injected larvae. (A) Confocal single-optical section images showing Tp1:H2B-mCherry (red) and Hnf4a (gray) expression in the liver. Arrows point to H2B-mCherry/Hnf4a double-positive cells. (B) A graph showing the percentage of H2B-mCherry+ cells among Hnf4a+ cells in the livers of the tomm22 MO-injected larvae. Red dots indicate the larvae shown in (A). (C) Confocal projection images showing the hepatic expression of ubb:mCherry (red, Cre-labeled cells) and Alcam (gray, BECs) at 9 dpf. 4-OHT was treated from 48 to 84 hpf. (D) Graph showing the percentage of ubb:mCherry+ hepatocytes, which were derived from BECs. fabp10a:CFP-NTR expression was used to define hepatocytes. Red marks indicate the larvae shown in (C). (E) Graph showing the percentage of mCherry+ cells among Alcam+ BECs at 9 dpf, indicating Cre-mediated labeling efficiency. n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry s931Tg ; s959Tg ; cz1701Tg chemical treatment: afimoxifene Days 7-13 hepatocyte IHC
s931Tg ; s959Tg ; cz1701Tg + MO1-tomm22 chemical treatment: afimoxifene Days 7-13 cholangiocyte IHC
Days 7-13 hepatocyte IHC
s939Tg control Day 6 cholangiocyte IHC
Day 6 Not Detected hepatocyte IHC
s939Tg + MO1-tomm22 standard conditions Day 5 cholangiocyte IHC
Day 5 hepatocyte IHC
Day 6 cholangiocyte IHC
Day 6 hepatocyte IHC
Days 7-13 cholangiocyte IHC
Days 7-13 hepatocyte IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC s931Tg ; s959Tg ; cz1701Tg chemical treatment: afimoxifene Days 7-13 cholangiocyte
IHC s931Tg ; s959Tg ; cz1701Tg + MO1-tomm22 chemical treatment: afimoxifene Days 7-13 cholangiocyte
Phenotype Details
Fish Conditions Stage Phenotype
s931Tg ; s959Tg ; cz1701Tg + MO1-tomm22 chemical treatment: afimoxifene Days 7-13 cholangiocyte mCherry expression increased amount, abnormal
s939Tg + MO1-tomm22 standard conditions Day 5 hepatocyte mCherry expression increased amount, abnormal
Day 6 hepatocyte mCherry expression increased amount, abnormal
Days 7-13 hepatocyte mCherry expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Gene Expression for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Gene Expr.