FIGURE

Fig. 3

ID

ZDB-FIG-170807-4

Publication

Wu et al., 2017 - tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae.

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Biliary epithelial cells (BECs) give rise to hepatocytes in tomm22 MO-injected larvae. (A) Confocal single-optical section images showing Tp1:H2B-mCherry (red) and Hnf4a (gray) expression in the liver. Arrows point to H2B-mCherry/Hnf4a double-positive cells. (B) A graph showing the percentage of H2B-mCherry⁺ cells among Hnf4a⁺ cells in the livers of the tomm22 MO-injected larvae. Red dots indicate the larvae shown in (A). (C) Confocal projection images showing the hepatic expression of ubb:mCherry (red, Cre-labeled cells) and Alcam (gray, BECs) at 9 dpf. 4-OHT was treated from 48 to 84 hpf. (D) Graph showing the percentage of ubb:mCherry⁺ hepatocytes, which were derived from BECs. fabp10a:CFP-NTR expression was used to define hepatocytes. Red marks indicate the larvae shown in (C). (E) Graph showing the percentage of mCherry⁺ cells among Alcam⁺ BECs at 9 dpf, indicating Cre-mediated labeling efficiency. n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.

Expression Data

Gene:	mCherry
Antibody:	zn-5
Fish:	s939Tg s939Tg + MO1-tomm22 cz1701Tg; s931Tg; s959Tg cz1701Tg; s931Tg; s959Tg + MO1-tomm22
Condition:	chemical treatment: afimoxifene
Knockdown Reagent:	MO1-tomm22
Anatomical Terms:	cholangiocyte hepatocyte
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s939Tg + MO1-tomm22 cz1701Tg; s931Tg; s959Tg + MO1-tomm22
Condition:	chemical treatment: afimoxifene
Knockdown Reagent:	MO1-tomm22
Observed In:	cholangiocyte hepatocyte
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Gene Expr.