FIGURE

Fig. 5

ID

ZDB-FIG-170807-6

Publication

Wu et al., 2017 - tomm22 Knockdown-Mediated Hepatocyte Damages Elicit Both the Formation of Hybrid Hepatocytes and Biliary Conversion to Hepatocytes in Zebrafish Larvae.

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Fig. 5

Suppression of Wnt/β-catenin signaling represses hepatocyte proliferation and the formation of hybrid hepatocytes in tomm22 MO-injected larvae. (A) Confocal single-optical section images showing WRE:d2GFP (green) and Tp1:H2B-mCherry (red) expression in the liver at 6 dpf. Arrows point to d2GFP/H2B-mCherry double-positive cells; arrowheads point to H2B-mCherry single-positive cells. Quantification of the percentage of d2GFP⁺ cells among H2B-mCherry⁺ cells is shown. (B) Scheme illustrating the period of XAV939 treatment for (C)–(F). (C) Confocal single-optical section images showing the hepatic expression of Tp1:H2B-mCherry (red) and Hnf4a (gray) at 7 dpf. Arrows point to H2B-mCherry/Hnf4a double-positive cells; arrowheads point to H2B-mCherry⁻/Hnf4a⁺ cells. Quantification of the percentage of H2B-mCherry⁻ cells among Hnf4a⁺ cells is shown. (D) Confocal single-optical images showing the hepatic expression of fabp10a:mAGFP-gmnn (green), Tp1:H2B-mCherry (red), and Hnf4a (gray) at 7 dpf. Arrows point to mAGFP-gmnn/H2B-mCherry/Hnf4a triple-positive cells; arrowheads point to mAGFP-gmnn/Hnf4a double-positive cells. (E) Graph showing the percentage of mAGFP-gmnn⁺ cells among Hnf4a⁺ cells. Red marks indicate the larvae shown in (D). (F) Graph showing the percentage of mCherry⁺ or mCherry⁻ proliferating hepatocytes. Red marks indicate the larvae shown in (D). n indicates the number of larvae examined. Scale bars: 20 μm; error bars: ±SEM.

Expression Data

Genes:	d2EGFP mAGFP mCherry
Fish:	s939Tg + MO1-tomm22 kyu1Tg; s939Tg kyu1Tg; s939Tg + MO1-tomm22 pt608Tg; s939Tg + MO1-tomm22
Condition:	chemical treatment by environment: XAV939
Knockdown Reagent:	MO1-tomm22
Anatomical Terms:	cholangiocyte hepatocyte liver
Stage Range:	Day 6 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s939Tg + MO1-tomm22 kyu1Tg; s939Tg + MO1-tomm22 pt608Tg; s939Tg + MO1-tomm22
Condition:	chemical treatment by environment: XAV939
Knockdown Reagent:	MO1-tomm22
Observed In:	cholangiocyte cholangiocyte Wnt signaling pathway hepatocyte hepatocyte cell population proliferation liver liver Wnt signaling pathway
Stage Range:	Day 6 to Days 7-13

Phenotype Detail

Acknowledgments

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