FIGURE

Fig. 2

ID

ZDB-FIG-170612-5

Publication

Peng et al., 2017 - Fatty Acid Oxidation in Zebrafish Adipose Tissue Is Promoted by 1α,25(OH)₂D₃.

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

cyp2r1 Gene Depletion in Zebrafish by TALENs

(A) The binding site of engineered TALENs on the cyp2r1 gene exon (E)1 (exons are in boxes). The underlined blue font indicates the sequences of the two targeting arms of TALENs, and the red font shows the restriction enzyme AluI cutting site.

(B) Mutation confirmation, as shown by the sequencing results of the transcripts of cyp2r1 gene of M1 and M2.

(D) An agarose gel electrophoresis image with the PCR products following AluI digestion of the cyp2r1 locus of F₂ fish. +/+, WT; +/−, heterozygous; −/−, homozygous.

(E) The relative expression levels of cyp2r1 in the adult livers of mutant lines and their control siblings. ^∗∗p < 0.01, compared with the control group.

(F and G) Comparison of the morphological features of the cyp2r1^−/− fish and wild-type controls at (F) 5 dpf and (G) 100 dpf.

See also Figure S2 and Tables S1 and S2.

Expression Data

Gene:	cyp2r1
Fish:	WT cyp2r1^{ihb152/ihb152} cyp2r1^{ihb153/ihb153}
Anatomical Term:	liver
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cyp2r1^{ihb152/ihb152} cyp2r1^{ihb153/ihb153}
Observed In:	liver whole organism
Stage Range:	Day 5 to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.