FIGURE

Fig. 3

ID

ZDB-FIG-170612-6

Publication

Peng et al., 2017 - Fatty Acid Oxidation in Zebrafish Adipose Tissue Is Promoted by 1α,25(OH)₂D₃.

Other Figures

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Fig. 3

Excessive VAT Developed in cyp2r1-Deficient Fish

(A) The neutral lipids of juvenile mutants and size-matched controls (both with a standard length of 7.6 mm) were stained with Nile Red and viewed with stereoscope (a1 and a2) and confocal microscope (a3 and a4).

(B and C) Shown here: (B) visceral adipocyte numbers and (C) area frequency of VAT in the confocal view (a3 and a4).

(D and E) Shown here: (D) Nile Red fluorescence and (E) cross-sections through trunks of adult mutants and their size-matched controls show increased subcutaneous AT (arrows) and excessive VAT (arrowheads) in the mutants. Scale bars, 1 mm.

(F) H&E-stained sections of VAT of adult mutants and their size-matched controls. Scale bars, 100 μm.

(G) Frequency distribution of adipocyte cell-surface area.

(H) The total lipid contents of males and females of both mutants and age-matched controls at 100 dpf. cyp2r1^+/+, n = 5; cyp2r1^−/−, n = 4.

Error bars indicate means ± SD. ^∗∗p < 0.01; N.S., no statistically significant difference.

See also Figure S3 and Table S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cyp2r1^{ihb153/ihb153}
Observed In:	female organism lipid male organism lipid pleuroperitoneal cavity fat cell subcutaneous fat visceral fat
Stage:	Adult

Phenotype Detail

Acknowledgments

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