ZFIN ID: ZDB-FIG-170519-30
Benz et al., 2016 - miR-19b Regulates Ventricular Action Potential Duration in Zebrafish. Scientific Reports   6:36033 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 Loss of miR-19b results in dysregulation of cardiac ion currents.

(a) qRT-PCR revealed dysregulation of various cardiac ion channels in miR-19b-deficient zebrafish. Transcripts with a predicted miR-19b binding site in its 3′-UTR are indicated in blue (±sd; n = 3 from 15 pooled embryos per sample; p < 0,05). (b) Illustration of a mammalian action potential with corresponding currents and genes. (c,d) Potential miR-19b targets were tested for direct regulation by a dual luciferase reporter gene assay. 3′UTR of indicated zebrafish (c) and human (d) genes was cloned downstream of a luciferase gene. Luciferase expression was normalized to renilla expression. As control, target sites were mutated to reverse miR-19b-induced repression. KCNE4, KCNJ2 and SCN1B proved to be direct targets in zebrafish and in humans. Additionally, the 3′UTR of human SCN4B showed responsiveness to miR-19b (±sd; n = 3; p < 0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kcne1 WT + MO2-mir19b standard conditions Long-pec whole organism RTPCR
kcne4 WT + MO2-mir19b standard conditions Long-pec whole organism RTPCR
kcnj2a WT + MO2-mir19b standard conditions Long-pec whole organism RTPCR
kcnj2b WT + MO2-mir19b standard conditions Long-pec whole organism RTPCR
scn1ba WT + MO2-mir19b standard conditions Long-pec whole organism RTPCR
scn4ba WT + MO2-mir19b standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-mir19b standard conditions Long-pec whole organism kcne1 expression increased amount, abnormal
Long-pec whole organism kcne4 expression increased amount, abnormal
Long-pec whole organism kcnj2a expression increased amount, abnormal
Long-pec whole organism kcnj2b expression increased amount, abnormal
Long-pec whole organism scn1ba expression increased amount, abnormal
Long-pec whole organism scn4ba expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.