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ZFIN ID: ZDB-FIG-170519-29
Benz et al., 2016 - miR-19b Regulates Ventricular Action Potential Duration in Zebrafish. Scientific Reports   6:36033 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4 Loss of miR-19b induces prolongation of the action potential

(a) Schematic illustration of an action potential with major depolarizing and repolarizing currents depicted. Terfenadine inhibits IKr current, thereby prolonging the ventricular action potential. (b) Atrial and ventricular m-mode recorded from control and MO19b-injected Tg(Myl7:GFP) zebrafish embryos after 30 μM Terfenadine treatment. (b,c) 25% ± 4.1% of miR-19b deficient embryos develop an AV-Block with the ventricle skipping every other contraction, whereas control injected embryos show no phenotype. (±sd; n = 3 with ≥10 animals per group; p < 0.05) (d,e) Patch-clamp Experiments revealed that morpholino-mediated loss of miR-19b results in prolonged action potentials with up to 57% ± 3.8% and 39% ± 3.5% prolongation of APD50 and APD90, respectively (±sd; n ≥ 10 from 5 hearts; p < 0.05). Additionally, the notch appeared to be less pronounced (stars).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg chemical treatment by environment: Terfenadine Long-pec atrium IFL
Long-pec cardiac ventricle IFL
twu34Tg + MO2-mir19b chemical treatment by environment: Terfenadine Long-pec atrium IFL
Long-pec cardiac ventricle IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-mir19b standard conditions Long-pec cardiac ventricle ventricular cardiac muscle cell action potential increased duration, abnormal
twu34Tg + MO2-mir19b chemical treatment by environment: Terfenadine Long-pec cardiac ventricle cardiac muscle contraction decreased frequency, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.